FILTAFLEX Ltd

PO Box 1224, Almonte, Ontario K0A 1A0, Canada
Tel: 613 256 3066; Fax: 613 256 8681




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Efficient membrane filtration by Spreadfilter

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Count colonies by HGMF Interpreter

Large-volume immunomagnetic concentrator

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HGMF Spreadfilter

HGMF SPREADFILTER for microbiological tests with membrane filters.

Don't waste time and labor cleaning and resterilizing contaminated filtration apparatus. By exploiting the ability of the HGMF's hydrophobic border to retain up to 10 mL of liquid the SPREADFILTER eliminates need for a funnel. Since the filtrant contacts only the HGMF the SPREADFILTER itself does not get contaminated - it does not need to be sterilized and you filter your next sample immediately. By eliminating cleanup work one SPREADFILTER replaces dozens of costly recyclable HGMF filters.

To use - place an HGMF on the SPREADFILTER, pipet inoculum in its center and use the pipet to spread it along one border. Spin the SPREADFILTER a quarter turn - spread again - twice more to cover the whole HGMF. Open the valve to pull liquid through, plate the HGMF and do the next sample. At the end of the day flush through well with 70% ethanol.
Notes:
- Long experience shows that organisms picked up occasionally from the frit by the underside of the HGMF have negligible effect on growths or counts on the HGMF. However, the glass frit and silicone rubber frame can be removed and sterilized, if desired.
- SPREADFILTERS are used by the USDA, CFIA and research and food industry labs around the world, and are cited in numerous publications (see REFERENCES below).
- The use of prefilters is unnecessary for most samples - if food suspensions contain very coarse debris that might bridge HGMF grid-cells, our FiltaTip pipet-tip prefilters are recommended.


Height 6 cm; diameter 12.5 cm; weight 900 g.
Cat. SF-1, price CAD$475.00 (shipping extra)
Order by fax or email please) - Email order or request more information....

REFERENCES
Rabaste, C. et al. 2007. The effects of handling and group size on welfare of pigs in lairage and their influence on stomach weight, carcass microbial contamination and meat quality. Can J. Animal Sci., 87: 3-12.
Ferens, W.A., et al. 2006. Intestinal Shiga Toxin-Producing Escherichia coli Bacteria Mitigate Bovine Leukemia Virus Infection in Experimentally Infected Sheep. Infect. Immun., 74: 2906-2916.
Canada Gazette, Vol 140, No. 10, 2006. Canadian Environmental Protection Act 1999 (Appendix to Ministerial Condition No. 13965 - Protocol for the Detection of Fungus Fusarium Species Strain CK 46-8 in Wastewater). Peterkin, P.I., and A.N. Sharpe. 1997. Methods MFHPB-26 (Enumeration of Escherichia coli by the Hydrophobic Grid-Membrane Filter (HGMF) Method), and MFHPB-17 (Enumeration of coliforms) in: Vol 2. Compendium of Analytical Methods Polyscience Publications Inc, Laval, Québec.
Charlebois, R., et al. 1991. Surface contamination of beef carcasses by fecal coliforms. J. Food Prot., 54: 950-956.
Fliss, I., et al. 1991. Microbiological quality of different fresh meat species in Tunisian slaughterhouses and markets. J. Food Prot., 54: 773-777.
Szabo, R., et al. 1990. Increased sensitivity of the rapid hydrophobic grid membrane filter enzyme-labeled antibody procedure for Escherichia coli O157 detection in foods and bovine feces. Appl. Environ. Microbiol., 56: 3546-3549.
Todd, E.C.D., et al. 1988. Rapid hydrophobic grid membrane filter-enzyme labeled antibody procedure for identification and enumeration of Escherichia coli O157 in foods. Appl. Environ. Microbiol., 54: 2536-2540.
Sharpe, A.N., et al. 1986. Re-usable filtration apparatus needs no sterilizing. Food Microbiol., 3: 243-246.
Updated Jul 2009